LIFR ELISA Kit, Rat

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LIFR ELISA Kit, Rat: 产品信息

产品名称
LIFR ELISA Kit, Rat
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
13 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Rat LIFR
干扰
Preparations of human LIFR ,LTF and mouse LIFR ,LTF at 50 ng/mL in a mid-range recombinant rat LIFR control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 679.00 1,310.00 2,553.00
SD 20.16 34.03 72.36
CV(%) 3.0% 2.6% 2.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 694.80 1,343.40 2,639.20
SD 23.65 65.31 103.07
CV(%) 3.4% 4.9% 3.9%
回收率
The recovery of Rat LIFR spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
88
Range
82 -91%
线性关系
Serum
  Recovery of detected
1:2 97%
1:4 103%
1:8 105%
1:16 98%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This LIFR ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat LIFR protein in Serum . It contains recombinant Rat LIFR, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

LIFR ELISA Kit, Rat: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Rat LIFR. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

LIFR 背景信息

LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp13, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (cardiotrophin like cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1 and cardiotrophin like cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF5) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.
全称
leukemia inhibitory factor receptor alpha
参考文献
  • Gearing, D.P. et al.,1991, EMBO J. 10 (10): 2839-2848.
  • Gearing, D.P. et al.,1992, New Biol. 4 (1): 61-65.
  • Mosley, B. et al.,1996, J. Biol. Chem. 271 (51): 32635-32643.
  • Timmermann, A. et al.,2002, Eur. J. Biochem. 269 (11): 2716-2726.
  • Lass, A. et al.,2002, Fertil. Steril. 76 (6): 1091-1096.
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