MCP-1/CCL2 ELISA Kit, Mouse

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MCP-1/CCL2 ELISA Kit, Mouse: 产品信息

产品名称
MCP-1/CCL2 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Cell supernatant
灵敏度
4.15 pg/mL
线性范围
13.28-850 pg/mL
特异性
Recognizes both recombinant and natural Mouse MCP-1/CCL2
干扰
Preparations a series of mouse CCL2 in a mid-range recombinant Human CCL7 control were assayed for interference. Interference was observed at concentrations 12.5 ng/mL.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 113.00 235.00 424.00
SD 11.91 6.25 13.90
CV(%) 9.5% 2.5% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 108.00 223.00 399.00
SD 6.43 10.44 29.69
CV(%) 5.9% 4.7% 7.4%
线性关系
Supernatant
  Recovery of detected
1:2 85%
1:4 95%
1:8 105%
1:16 103%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This MCP-1/CCL2 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MCP-1/CCL2 protein in Cell supernatant . It contains recombinant Mouse MCP-1/CCL2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

MCP-1/CCL2 ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse CCL2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 6.0% with 25 ng/mL of recombinant Human CCL7.

MCP-1/CCL2 ELISA Kit, Mouse: 别称

AI323594 ELISA Kit, Mouse; HC11 ELISA Kit, Mouse; JE ELISA Kit, Mouse; MCAF ELISA Kit, Mouse; MCP-1 ELISA Kit, Mouse; MCP1 ELISA Kit, Mouse; Scya2 ELISA Kit, Mouse; Sigje ELISA Kit, Mouse; SMC-CF ELISA Kit, Mouse

MCP-1/CCL2 背景信息

Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection [7–9]. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
全称
chemokine (C-C motif) ligand 2
研究领域
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Vásquez-Loarte T, Trubnykova M, Guio H. Genetic association meta-analysis: a new classification to assess ethnicity using the association of MCP-1 -2518 polymorphism and tuberculosis susceptibility as a model. BMC Genetics. 2015;16:128.
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