MICB ELISA Kit, Human

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MICB ELISA Kit, Human: 产品信息

产品名称
MICB ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
16.6 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Human MICB
干扰
Preparations of human MICA and BST1-10H-NKG2D at 40 ng/mL in a mid-range recombinant human MICB control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 882.00 1,762.00 3,518.00
SD 0.06 0.04 0.13
CV(%) 7.0% 2.4% 3.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 958.00 1,826.00 3,418.00
SD 0.05 0.08 0.08
CV(%) 5.5% 5.5% 2.5%
回收率
The recovery of Human MICB spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
99
Range
96-101%
线性关系
Serum
  Recovery of detected
1:2 94%
1:4 106%
1:8 116%
1:16 109%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This MICB ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human MICB protein in Serum . It contains recombinant Human MICB, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

MICB ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human MICB. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

MICB ELISA Kit, Human: 别称

PERB11.2 ELISA Kit, Human

MICB 背景信息

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
全称
MHC class I polypeptide-related sequence B
参考文献
  • Bauer, S. et al., 1999, Science. 285:727-729.
  • Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
  • Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
  • Stephens, H., 2001, Trends Immunol. 22: 378-385.
  • Borrego, F. et al., 2002, Mol. Immunol. 38: 637–660.
  • Groh, V. et al., 2002, Nature. 419:734-738.
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