MMP-8 ELISA Kit, Human

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MMP-8 ELISA Kit, Human: 产品信息

产品名称
MMP-8 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
17.6 pg/mL
线性范围
43.75-2800 pg/mL
特异性
Recognizes both recombinant and natural Human MMP-8
干扰
Preparations of the factors listed below at 10 ng/mL in a mid-range recombinant human MMP8 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 48.00 96.00 177.00
SD 0.00 0.00 0.01
CV(%) 5.1% 2.4% 4.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 156.00 304.00 556.00
SD 0.01 0.01 0.02
CV(%) 4.5% 4.4% 4.3%
回收率
The recovery of Human MMP8 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
96
Range
91-99%
线性关系
Serum
  Recovery of detected
1:2 92%
1:4 105%
1:8 107%
1:16 96%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This MMP-8 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP-8 protein in Serum . It contains recombinant Human MMP-8, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

MMP-8 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human MMP8.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

MMP-8 ELISA Kit, Human: 别称

CLG1 ELISA Kit, Human; HNC ELISA Kit, Human; MMP-8 ELISA Kit, Human; PMNL-CL ELISA Kit, Human

MMP-8 背景信息

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Neutrophil collagenase, also known as Matrix metalloproteinase-8, MMP-8, and CLG1, is a member of the peptidase M1A family. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 in the tumour may have a protective effect against lymph node metastasis. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.
全称
matrix metallopeptidase 8
参考文献
  • Hasty K.A., et al.,(1990), Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J. Biol. Chem. 265:11421-11424.
  • Blaeser J., et al., (1991), Mercurial activation of human polymorphonuclear leucocyte procollagenase.Eur. J. Biochem. 202:1223-1230.
  • Blaeser J., et al.,(1991), Mercurial activation of human polymorphonuclear leucocyte procollagenase. Eur. J. Biochem. 202:1223-1230.
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