NBL1 ELISA Kit, Human

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NBL1 ELISA Kit, Human: 产品信息

产品名称
NBL1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
11.2 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human NBL1
干扰
Preparations of BMP2 at 30 ng/mL in a mid-range recombinant human DAN control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.41 0.87 1.55
SD 0.01 0.01 0.04
CV(%) 1.9% 1.3% 2.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.40 0.77 1.56
SD 0.02 0.01 0.04
CV(%) 3.9% 1.6% 2.4%
回收率
The recovery of Human NBL1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
116.773854069224
Range
113-120%
线性关系
Serum
  Recovery of detected
1:2 105%
1:4 109%
1:8 97%
1:16 88%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This NBL1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 protein in Serum . It contains recombinant Human NBL1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

NBL1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human DAN. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of BMP2 at 30 ng/mL in a mid-range recombinant human DAN control were assayed for interference. No significant interference was observed.

NBL1 ELISA Kit, Human: 别称

D1S1733E ELISA Kit, Human; DAN ELISA Kit, Human; DAND1 ELISA Kit, Human; NB ELISA Kit, Human; NO3 ELISA Kit, Human

NBL1 背景信息

The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N3) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.
全称
neuroblastoma 1, DAN family BMP antagonist
参考文献
  • Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.
  • Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.
  • Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.
  • Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.
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