Nectin-2 ELISA Kit, Human

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Nectin-2 ELISA Kit, Human: 产品信息

产品名称
Nectin-2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
7.95 pg/mL
线性范围
23.4-1500 pg/mL
特异性
Recognizes both recombinant and natural Human Nectin-2
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 230.57 549.40 913.97
SD 8.33 18.79 31.20
CV(%) 3.6% 3.4% 3.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 254.20 582.20 942.92
SD 17.14 18.74 91.60
CV(%) 6.7% 3.2% 9.7%
回收率
The recovery of Human NECTIN2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=2)
Average % Recovery
84
Range
81 -87%
线性关系
Serum
  Recovery of detected
1:2 90%
1:4 94%
1:8 106%
1:16 92%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Nectin-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Nectin-2 protein in Serum . It contains recombinant Human Nectin-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Nectin-2 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Nectin-2 ELISA Kit, Human: 别称

CD112 ELISA Kit, Human; HVEB ELISA Kit, Human; Nectin-2 ELISA Kit, Human; PRR2 ELISA Kit, Human; PVRR2 ELISA Kit, Human

Nectin-2 背景信息

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
全称
poliovirus receptor-related 2 (herpesvirus entry mediator B)
参考文献
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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