NOV/CCN3 ELISA Kit, Human

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NOV/CCN3 ELISA Kit, Human: 产品信息

产品名称
NOV/CCN3 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
55.91 pg/mL
线性范围
93.75-6000 pg/mL
特异性
Recognizes both recombinant and natural Human NOV/CCN3
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 941.82 1,927.88 4,119.95
SD 43.23 67.38 217.04
CV(%) 4.6% 3.5% 5.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 953.04 2,091.91 4,073.22
SD 76.15 188.89 262.62
CV(%) 8.0% 9.0% 6.4%
回收率
The recovery of Human NOV spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
87
Range
82 -91%
线性关系
Serum
  Recovery of detected
1:2 118%
1:4 104%
1:8 96%
1:16 70%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This NOV/CCN3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human NOV/CCN3 protein in Serum . It contains recombinant Human NOV/CCN3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

NOV/CCN3 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human NOV. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

NOV/CCN3 ELISA Kit, Human: 别称

CCN3 ELISA Kit, Human; IBP-9 ELISA Kit, Human; IGFBP-9 ELISA Kit, Human; IGFBP9 ELISA Kit, Human; NOVh ELISA Kit, Human

NOV/CCN3 背景信息

Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development , and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis and a crucial component within the bone marrow microenvironment.
全称
nephroblastoma overexpressed
参考文献
  • Manara MC, et al. (2002) The expression of ccn3(nov) gene in musculoskeletal tumors. Am J Pathol. 160(3): 849-59.
  • Lin CG, et al. (2003) CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family. J Biol Chem. 278(26): 24200-8.
  • Vallacchi V, et al. (2009) CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. Cancer Res. 68(3): 715-23.
  • Sin WC, et al. (2009) Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization. J Biol Chem. 284(43): 29935-44.
  • McCallum L, et al. (2009) CCN3--a key regulator of the hematopoietic compartment. Blood Rev. 23(2): 79-85.
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