P-Selectin ELISA Kit, Human

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P-Selectin ELISA Kit, Human: 产品信息

产品名称
P-Selectin ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
10.91 pg/mL
线性范围
50-3200 pg/mL
特异性
Recognizes both recombinant and natural Human P-Selectin
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 707.00 1,495.00 2,729.00
SD 19.36 39.06 93.74
CV(%) 2.7% 2.6% 3.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 665.00 1,378.00 2,607.00
SD 49.58 95.48 164.06
CV(%) 7.5% 6.9% 6.3%
回收率
The recovery of Human SELP spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
91
Range
88 -95%
线性关系
Serum
  Recovery of detected
1:1 96%
1:2 104%
1:4 104%
1:8 96%
Supernatant
  Recovery of detected
1:1 93%
1:2 98%
1:4 109%
1:8 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This P-Selectin ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human P-Selectin protein in Serum . It contains recombinant Human P-Selectin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

P-Selectin ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human SELP. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

P-Selectin ELISA Kit, Human: 别称

CD62 ELISA Kit, Human; CD62P ELISA Kit, Human; GMP140 ELISA Kit, Human; GRMP ELISA Kit, Human; LECAM3 ELISA Kit, Human; P-Selectin ELISA Kit, Human; PADGEM ELISA Kit, Human; PSEL ELISA Kit, Human

P-Selectin 背景信息

P selectin (SELP) is a 14kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
全称
selectin P (granule membrane protein 140kDa, antigen CD62)
参考文献
  • Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.
  • Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.
  • Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.
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