PCSK9 ELISA Kit, Mouse

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PCSK9 ELISA Kit, Mouse: 产品信息

产品名称
PCSK9 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
47.09 pg/mL
线性范围
125-8000 pg/mL
特异性
Recognizes both recombinant and natural Mouse PCSK9
干扰
Preparations of mouse LDLR at 30 ng/mL in a mid-range recombinant mouse PCSK9 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 496.66 986.39 2,040.14
SD 25.11 47.54 76.26
CV(%) 5.1% 4.8% 3.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 505.93 993.25 2,061.85
SD 36.93 56.17 152.15
CV(%) 7.3% 5.7% 7.4%
回收率
The recovery of Mouse PCSK9 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
77
Range
72-80%
线性关系
Serum
  Recovery of detected
1:2 106%
1:4 87%
1:8 77%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Sample Dilution Buffer Concentrate
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
产品概述
This PCSK9 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PCSK9 protein in Serum . It contains recombinant Mouse PCSK9, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PCSK9 ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural mouse PCSK9. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PCSK9 ELISA Kit, Mouse: 别称

AI415265 ELISA Kit, Mouse; AI747682 ELISA Kit, Mouse; FH3 ELISA Kit, Mouse; HCHOLA3 ELISA Kit, Mouse; Narc1 ELISA Kit, Mouse; PC9 ELISA Kit, Mouse

PCSK9 背景信息

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

References

全称
proprotein convertase subtilisin/kexin type 9
参考文献
  • Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
  • Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
  • Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
  • Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
  • Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
  • Di'ao Xinxuekang Capsule, a Chinese Medicinal Product, Decreases Serum Lipids Levels in High-Fat Diet-Fed ApoE–/–Mice by Downregulating PCSK9
    Author
    Qu, L;Li, D;Gao, X;Li, Y;Wu, J;Zou, W;
    Year
    2018
    Journal
    Frontiers in pharmacology
    Application
    ELISA (serum)
  • A small-molecule inhibitor of PCSK9 transcription ameliorates atherosclerosis through the modulation of FoxO1/3 and HNF1α
    Author
    Wang, X;Chen, X;Zhang, X;Su, C;Yang, M;He, W;Du, Y;Si, S;Wang, L;Hong, B;
    Year
    2020
    Journal
    EBioMedicine
    Application
    ELISA
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