PD-1 ELISA Kit, Human

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PD-1 ELISA Kit, Human: 产品信息

产品名称
PD-1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
1.57 pg/mL
线性范围
3.91-250 pg/mL
特异性
Recognizes both recombinant and natural Human PD-1
干扰
Preparations of the factors listed below at 10 ng/mL in a mid-range recombinant human PD1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 48.00 87.00 182.00
SD 1.65 8.08 7.77
CV(%) 3.4% 9.3% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 49.00 101.00 191.00
SD 4.16 12.29 8.33
CV(%) 8.4% 12.2% 4.4%
回收率
The recovery of Human PDCD1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
103
Range
100 -105%
线性关系
Serum
  Recovery of detected
1:1 92%
1:2 103%
1:4 97%
1:8 75%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This PD-1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human PD-1 protein in Serum . It contains recombinant Human PD-1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PD-1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human PD1. The factors listed below were prepared at 10 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PD-1 ELISA Kit, Human: 别称

CD279 ELISA Kit, Human; hPD-1 ELISA Kit, Human; hPD-l ELISA Kit, Human; hSLE1 ELISA Kit, Human; PD-1 ELISA Kit, Human; PD1 ELISA Kit, Human; SLEB2 ELISA Kit, Human

PD-1 背景信息

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-1 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
全称
programmed cell death 1
参考文献
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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