PDGFRA ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

PDGFRA ELISA Kit, Human: 产品信息

产品名称
PDGFRA ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
86.73 pg/mL
线性范围
109.38-7000 pg/mL
特异性
Recognizes both recombinant and natural Human PDGFRA
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,237.59 2,512.69 5,158.21
SD 72.70 109.99 245.24
CV(%) 5.9% 4.4% 4.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,135.39 2,464.60 4,891.08
SD 77.92 200.67 160.46
CV(%) 6.9% 8.1% 3.3%
回收率
The recovery of Human PDGFRA spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
90
Range
83-98%
线性关系
Serum
  Recovery of detected
1:2 100%
1:4 112%
1:8 107%
1:16 96%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This PDGFRA ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGFRA protein in Serum . It contains recombinant Human PDGFRA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PDGFRA ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human PDGFRA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PDGFRA ELISA Kit, Human: 别称

CD140A ELISA Kit, Human; PDGFR-2 ELISA Kit, Human; PDGFR2 ELISA Kit, Human; RHEPDGFRA ELISA Kit, Human

PDGFRA 背景信息

PDGFRA, also known as CD14a, together with the structurally homolog protein PDGFRB (CD14b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.
全称
platelet-derived growth factor receptor, alpha polypeptide
参考文献
  • Oseini AM, et al. (2009) PDGFRalpha: a new therapeutic target in the treatment of hepatocellular carcinoma? Expert Opin Ther Targets. 13(4): 443-54.
  • Meister M, et al. (2009) Expression and mutational status of PDGFR in thymic tumours. Anticancer Res. 29(10): 4057-61.
  • Martinho O, et al. (2009) Expression, mutation and copy number analysis of platelet-derived growth factor receptor A (PDGFRA) and its ligand PDGFA in gliomas. Br J Cancer. 101(6): 973-82.
  • Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions
    Author
    Mu, Y;Wu, X;Hao, Z;
    Year
    2018
    Journal
    BMC Cell Biol.
    Application
    ELISA
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.