PIGR ELISA Kit, Mouse

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PIGR ELISA Kit, Mouse: 产品信息

产品名称
PIGR ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
3.72 pg/mL
线性范围
6.25-400 pg/mL
特异性
Recognizes both recombinant and natural Mouse PIGR
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 65.00 115.00 242.00
SD 2.59 5.18 11.53
CV(%) 4.0% 4.5% 4.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 71.00 142.00 281.00
SD 3.74 17.74 24.60
CV(%) 5.3% 12.5% 8.8%
回收率
The recovery of Mouse PIGR spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
86
Range
77 -90%
线性关系
Serum
  Recovery of detected
1:1 84%
1:2 98%
1:4 102%
1:8 128%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This PIGR ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PIGR protein in Serum . It contains recombinant Mouse PIGR, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PIGR ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse PIGR. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PIGR ELISA Kit, Mouse: 别称

Pigr ELISA Kit, Mouse

PIGR 背景信息

Polymeric immunoglobulin receptor, also known asPIGR, is a member of the immunoglobulin superfamilyand aFc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion ofIgAandIgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.
全称
polymeric immunoglobulin receptor
参考文献
  • Coyne, R. S. et al., 1995, J. Biol. Chem. 269 (50) :31620-31625. 
  • Kaetzel,C.S., 2001,  Curr Biol. 11(1): R35-38.
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