PLA2G1B ELISA Kit, Mouse

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

PLA2G1B ELISA Kit, Mouse: 产品信息

产品名称
PLA2G1B ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
0.66 pg/mL
线性范围
2.82-180 pg/mL
特异性
Recognizes both recombinant and natural Mouse PLA2G1B
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 25.36 55.11 106.27
SD 1.02 1.71 4.43
CV(%) 4.0% 3.1% 4.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 26.04 58.08 106.90
SD 1.49 3.22 10.03
CV(%) 5.7% 5.5% 9.4%
回收率
The recovery of Mouse PLA2G1B spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
90
Range
88-91%
线性关系
Serum
  Recovery of detected
1:2 79%
1:4 96%
1:8 102%
1:16 124%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This PLA2G1B ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PLA2G1B protein in Serum . It contains recombinant Mouse PLA2G1B, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

PLA2G1B ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse PLA2G1B. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

PLA2G1B ELISA Kit, Mouse: 别称

Pla2a ELISA Kit, Mouse; sPLA2IB ELISA Kit, Mouse

PLA2G1B 背景信息

phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to thephospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.
全称
phospholipase A2, group IB (pancreas)
参考文献
  • Labonté,E.D. et al., 2006, Diabetes. 55 (4) :935-41.
  • Mounier,C.M. et al.,2008, Br J Cancer. 98 (3):587-95.
  • Hui,D.Y. et al., 2009, Br J Pharmacol. 157 (7):1263-9.
  • Labonté,E.D. et al., 2010, FASEB J. 24 (7):2516-24.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.