SAA4 ELISA Kit, Human

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SAA4 ELISA Kit, Human: 产品信息

产品名称
SAA4 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
7.14 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human SAA4
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 494.00 1,014.00 2,100.00
SD 0.01 0.02 0.10
CV(%) 1.8% 1.7% 4.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 532.00 1,071.00 2,083.00
SD 26.02 57.89 48.68
CV(%) 4.9% 5.4% 2.3%
回收率
The recovery of Human SAA4 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
88
Range
88 -89%
线性关系
Serum
  Recovery of detected
1:2 96%
1:4 100%
1:8 103%
1:16 101%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This SAA4 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human SAA4 protein in Serum . It contains recombinant Human SAA4, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

SAA4 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human SAA4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

SAA4 ELISA Kit, Human: 别称

C-SAA ELISA Kit, Human; CSAA ELISA Kit, Human; SAA4 ELISA Kit, Human

SAA4 背景信息

SAA4 is a member of the SAA family.SAA proteins are family of apolipoproteins of high density lipoprotein (HDL). They can be separated into two distinct groups. First group (SAA1, SAA2, and SAA3) consists of acute phase reactant whose expression level increase in the blood in a response to trauma, infection, inflammation, and neoplasia. These acute phase SAAs associates with HDL during inflammation and remodel the HDL particle by displacing Apo-A1. The second distinct group consists of SAA4 and SAA5 which exist as the minor apolipoproteins on HDL, but this group of SAA constitutes more than 9% of all the SAA during homeostasis, and it is thought to play a role in the normal functioning of the HDL particle. SAA4 is a constitutively expressed protein which expressed only in humans and mice.It is connected almost completely with lipoproteins of the high density range. The physiological function of SAA4 is unknown, and its serum concentration has no association with those of other major apolipoproteins.
全称
serum amyloid A4, constitutive
参考文献
  • Davila S, et al. (2010) New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun. 11(3):232-8.
  • Murphy CL, et al. (2009) AA amyloidosis associated with a mutated serum amyloid A4 protein. Mol Med. Amyloid. 16(2):84-8.
  • Prakash T, et al. (2010) Expression of conjoined genes: another mechanism for gene regulation in eukaryotes. PLoS One. 5(10):e13284.
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