SerpinA1 ELISA Kit, Human

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SerpinA1 ELISA Kit, Human: 产品信息

产品名称
SerpinA1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
26.37 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Human SerpinA1
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 471.00 1,016.00 1,878.00
SD 13.30 30.30 55.34
CV(%) 2.8% 3.0% 2.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 477.00 1,024.00 2,021.00
SD 9.82 39.53 115.60
CV(%) 2.1% 3.9% 5.7%
回收率
The recovery of Human SerpinA1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
87
Range
79 -91%
线性关系
Serum
  Recovery of detected
1:2 95%
1:4 100%
1:8 124%
1:16 76%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This SerpinA1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human SerpinA1 protein in Serum . It contains recombinant Human SerpinA1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

SerpinA1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human SERPINA1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

SerpinA1 ELISA Kit, Human: 别称

A1A ELISA Kit, Human; A1AT ELISA Kit, Human; AAT ELISA Kit, Human; alpha1AT ELISA Kit, Human; MGC23330 ELISA Kit, Human; MGC9222 ELISA Kit, Human; PI ELISA Kit, Human; PI1 ELISA Kit, Human; PRO2275 ELISA Kit, Human; SerpinA1 ELISA Kit, Human

SerpinA1 背景信息

SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. It association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and a complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP). Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.
全称
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
参考文献
  • Khnlein T, et al. (2008) Alpha-1 antitrypsin deficiency: pathogenesis, clinical presentation, diagnosis, and treatment. Am J Med. 121(1): 3-9.
  • Camelier AA, et al. (2008) Alpha-1 antitrypsin deficiency: diagnosis and treatment. J Bras Pneumol. 34(7): 514-27.
  • Subramaniyam D, et al. (2010) Cholesterol rich lipid raft microdomains are gateway for acute phase protein, SERPINA1. Int J Biochem Cell Biol. 42(9): 1562-70.
  • Kilty SJ, et al. (2010) Polymorphisms in the SERPINA1 (Alpha-1-Antitrypsin) gene are associated with severe chronic rhinosinusitis unresponsive to medical therapy. Am J Rhinol Allergy. 24(1): e4-9.
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