Alpha 2 Antiplasmin/SerpinF2 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10297
Alpha 2 Antiplasmin/SerpinF2 ELISA Kit, Human: 产品信息
产品名称
Alpha 2 Antiplasmin/SerpinF2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
7 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human Alpha 2 Antiplasmin/SerpinF2
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 540.83 | 1,104.86 | 2,139.29 |
| SD | 28.55 | 54.43 | 118.09 |
| CV(%) | 5.3% | 4.9% | 5.5% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 510.13 | 1,089.88 | 2,006.65 |
| SD | 34.50 | 61.96 | 123.81 |
| CV(%) | 6.8% | 5.7% | 6.2% |
回收率
The recovery of Human SerpinF2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 80 |
| Range |
| 74-84% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 99% |
| 1:4 | 107% |
| 1:8 | 102% |
| 1:16 | 92% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Alpha 2 Antiplasmin/SerpinF2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Alpha 2 Antiplasmin/SerpinF2 protein in Serum . It contains recombinant Human Alpha 2 Antiplasmin/SerpinF2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
Alpha 2 Antiplasmin/SerpinF2 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human SERPINF2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Alpha 2 Antiplasmin/SerpinF2 ELISA Kit, Human: 别称
A2AP ELISA Kit, Human; AAP ELISA Kit, Human; ALPHA-2-PI ELISA Kit, Human; API ELISA Kit, Human; PLI ELISA Kit, Human
Alpha 2 Antiplasmin/SerpinF2 背景信息
SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta also express this protein at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.
全称
serpin peptidase inhibitor, clade F, member 2
参考文献
- Lee KN, et al. (2004) Alpha2-antiplasmin: potential therapeutic roles in fibrin survival and removal. Curr Med Chem Cardiovasc Hematol Agents. 2(4): 303-10.
- Matsuno H. (2006) Alpha2-antiplasmin on cardiovascular diseases. Curr Pharm Des. 12(7): 841-7.
- Burnouf T, et al. (2007) Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 35(4): 349-53.
- Carpenter SL, et al. (2008) Alpha2-antiplasmin and its deficiency: fibrinolysis out of balance. Haemophilia. 14(6): 1250-4.
