SLAM/CD150 ELISA Kit, Human

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SLAM/CD150 ELISA Kit, Human: 产品信息

产品名称
SLAM/CD150 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
2.9 pg/mL
线性范围
10.94-700 pg/mL
特异性
Recognizes both recombinant and natural Human SLAM/CD150
干扰
Preparations of SH2D1A、ARF5、ANTXR1、PTPRC、LGALS1 and GK at 7 ng/mL in a mid-range recombinant human SLAMF1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.13 0.23 0.50
SD 0.01 0.02 0.03
CV(%) 7.6% 6.9% 6.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.12 0.24 0.51
SD 0.01 0.01 0.03
CV(%) 4.4% 4.1% 6.4%
回收率
The recovery of Human SLAMF1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
101.23986906789399
Range
101%-102%
线性关系
Supernatant
  Recovery of detected
1:2 107%
1:4 99%
1:8 94%
1:16 102%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This SLAM/CD150 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human SLAM/CD150 protein in Recombinant protein . It contains recombinant Human SLAM/CD150, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

SLAM/CD150 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant human SLAMF1. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of SH2D1A、ARF5、ANTXR1、PTPRC、LGALS1 and GK at 7 ng/mL in a mid-range recombinant human SLAMF1 control were assayed for interference. No significant interference was observed.

SLAM/CD150 ELISA Kit, Human: 别称

CD150 ELISA Kit, Human; CDw150 ELISA Kit, Human; SLAM ELISA Kit, Human

SLAM/CD150 背景信息

CD15/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. The CD15 receptor is expressed on thymocytes, activated and memory T cells, B cells, platelets, natural killer T cells, and mature dendritic cells, and is also detected on tumor cells of Hodgkin's lymphoma (HL) and diffuse large B-cell lymphoma with an activated B cell phenotype. Additionally, it is the immune cell receptor for measles virus (MV). As a self-ligand, CD15 performs diverse immunologic functions including T/B-cell costimulation, induction of IFN-&gamma in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th phenotype, and inhibition of apoptosis in B cells. Furthermore, CD15 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.
全称
signaling lymphocytic activation molecule family member 1
参考文献
  • Tatsuo H, et al. (2002) The morbillivirus receptor SLAM (CD150). Microbiol Immunol. 46(3): 135-42.
  • Sidorenko SP, et al. (2003)The dual-function CD150 receptor subfamily: the viral attraction. Nat Immunol. 4(1): 19-24.
  • Yurchenko MY, et al. (2010) CD150 regulates JNK1/2 activation in normal and Hodgkin's lymphoma B cells. Immunol Cell Biol. 88(5): 565-74.
  • Leonard VH, et al. (2010) Measles virus selectively blind to signaling lymphocytic activation molecule (SLAM ; CD150) is attenuated and induces strong adaptive immune responses in rhesus monkeys. J Virol. 84(7): 3413-20.
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