ST2/IL-1 RL1 ELISA Kit, Human

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ST2/IL-1 RL1 ELISA Kit, Human: 产品信息

产品名称
ST2/IL-1 RL1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
6.22 pg/mL
线性范围
12.5-800 pg/mL
特异性
Recognizes both recombinant and natural Human ST2/IL-1 RL1
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 117.40 245.01 490.52
SD 8.07 16.38 18.10
CV(%) 6.9% 6.7% 3.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 121.22 245.79 489.25
SD 8.26 13.24 17.25
CV(%) 6.8% 5.4% 3.5%
回收率
The recovery of Human IL1RL1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
74
Range
70 -79%
线性关系
Serum
  Recovery of detected
1:2 105%
1:4 101%
1:8 96%
1:16 90%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This ST2/IL-1 RL1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human ST2/IL-1 RL1 protein in Serum . It contains recombinant Human ST2/IL-1 RL1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

ST2/IL-1 RL1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL1R4. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

ST2/IL-1 RL1 ELISA Kit, Human: 别称

DER4 ELISA Kit, Human; FIT-1 ELISA Kit, Human; IL1RL1 ELISA Kit, Human; IL33R ELISA Kit, Human; IL‑1 R4 ELISA Kit, Human; ST2 ELISA Kit, Human; ST2L ELISA Kit, Human; ST2V ELISA Kit, Human; T1 ELISA Kit, Human

ST2/IL-1 RL1 背景信息

IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interlukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described, and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage pro-inflammatory function. IL33 is a specific ligand of ST2L and induces production of Th2 cytokines.
全称
interleukin 1 receptor-like 1
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
参考文献
  • Savenije OE, et al. (2011) Interleukin-1 receptor-like 1 polymorphisms are associated with serum IL1RL1-a, eosinophils, and asthma in childhood. J Allergy Clin Immunol. 127(3): 750-6.
  • Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics. 67(3): 284-90.
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