TARC ELISA Kit, Human
Cat: KIT10233
TARC ELISA Kit, Human: 产品信息
产品名称
TARC ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
9.45 pg/mL
线性范围
18.75-1200 pg/mL
特异性
Recognizes both recombinant and natural Human TARC
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 65.00 | 178.00 | 449.00 |
| SD | 6.26 | 13.25 | 9.02 |
| CV(%) | 9.7% | 7.5% | 2.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 142.00 | 301.00 | 626.00 |
| SD | 13.87 | 17.67 | 62.01 |
| CV(%) | 9.7% | 5.9% | 9.9% |
回收率
The recovery of Human CCL17 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 98% |
| 1:4 | 92% |
| 1:8 | 110% |
| 1:16 | 120% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This TARC ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TARC protein in Serum . It contains recombinant Human TARC, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
TARC ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CCL17. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
TARC ELISA Kit, Human: 别称
A-152E5.3 ELISA Kit, Human; ABCD-2 ELISA Kit, Human; SCYA17 ELISA Kit, Human; TARC ELISA Kit, Human
TARC 背景信息
Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokin ligand 17 (CCL17), also known as thymus and activation regulated chemokine(TARC), is a small cytokine belonging to the C-C chemokine family. CCL17 is expressed maily in thymus and transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. CCL17 can induce chemotaxis in T cells by binding with the chemokine receptor CCR4.
全称
chemokine (C-C motif) ligand 17
参考文献
- Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.
- Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.
- Morita A, et al. (2002). Evaluation of human thymus and activation-regulated chemokine concentrations in blood using a new sandwich ELISA based on monoclonal antibodies. Clin Chim Acta. 322 (1-2): 67-75.
