TFPI ELISA Kit, Mouse

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TFPI ELISA Kit, Mouse: 产品信息

产品名称
TFPI ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
13.6 pg/mL
线性范围
18.8-1200 pg/mL
特异性
Recognizes both recombinant and natural Mouse TFPI
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.20 0.39 0.73
SD 0.01 0.02 0.03
CV(%) 3.2% 4.4% 4.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.20 0.40 0.79
SD 0.01 0.03 0.06
CV(%) 6.0% 7.9% 8.1%
回收率
The recovery of Mouse TFPI spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
123
Range
121-126%
线性关系
Supernatant
  Recovery of detected
1:2 103%
1:4 97%
1:8 97%
1:16 89%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This TFPI ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TFPI protein in Recombinant protein . It contains recombinant Mouse TFPI, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TFPI ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant mouse TFPI. The factors listed above were prepared at 900 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TFPI ELISA Kit, Mouse: 别称

A630013F22Rik ELISA Kit, Mouse; AW552122 ELISA Kit, Mouse; EPI ELISA Kit, Mouse; LACI ELISA Kit, Mouse

TFPI 背景信息

Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.
全称
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
参考文献
  • Lwaleed BA, et al. (2006) Tissue factor pathway inhibitor: structure, biology and involvement in disease. J Pathol. 208(3): 327-39.
  • Amirkhosravi A, et al. (2007) The role of tissue factor pathway inhibitor in tumor growth and metastasis. Semin Thromb Hemost. 33(7): 643-52.
  • Maroney SA, et al. (2008) Expression of tissue factor pathway inhibitor by endothelial cells and platelets. Transfus Apher Sci. 38(1): 9-14.
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