TIM-3 ELISA Kit, Human

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TIM-3 ELISA Kit, Human: 产品信息

产品名称
TIM-3 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
9.29 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Human TIM-3
干扰
Preparations of GST-Xa-Ab1/42 at 20 ng/mL in a mid-range recombinant human TIMD3 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.30 0.66 1.39
SD 0.01 0.03 0.04
CV(%) 4.6% 4.3% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.26 0.60 1.29
SD 0.02 0.06 0.09
CV(%) 7.4% 9.9% 7.2%
回收率
The recovery of Human HAVCR2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
112
Range
94-128%
线性关系
Serum
  Recovery of detected
1:2 99%
1:4 96%
1:8 96%
1:16 82%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This TIM-3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TIM-3 protein in Serum . It contains recombinant Human TIM-3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TIM-3 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TIMD3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TIM-3 ELISA Kit, Human: 别称

CD366 ELISA Kit, Human; HAVcr-2 ELISA Kit, Human; HAVCR2 ELISA Kit, Human; KIM-3 ELISA Kit, Human; Tim-3 ELISA Kit, Human; TIM3 ELISA Kit, Human; TIMD-3 ELISA Kit, Human; TIMD3 ELISA Kit, Human

TIM-3 背景信息

Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both mice and human. Recently, identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In tumor rejection model, soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
全称
hepatitis A virus cellular receptor 2
研究领域
参考文献
  • Geng H, et al. (2006) Soluble form of T cell Ig mucin 3 is an inhibitory molecule in T cell-mediated immune response. J Immunol. 176(3): 1411-20.
  • Anderson AC, et al. (2006) TIM-3 in autoimmunity. Curr Opin Immunol. 18(6): 665-9.
  • Anderson DE. (2007) TIM-3 as a therapeutic target in human inflammatory diseases. Expert Opin Ther Targets. 11(8): 1005-9.
  • Pan HF, et al. (2010) TIM-3 as a new therapeutic target in systemic lupus erythematosus. Mol Biol Rep. 37(1): 395-8.
  • Soluble Tim3 detection by time-resolved fluorescence immunoassay and its application in membranous nephropathy
    Author
    Chen, M;Wang, L;Wang, Y;Zhou, X;Liu, X;Chen, H;Huang, B;Hu, Z;
    Year
    2020
    Journal
    J. Clin. Lab. Anal.
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