TNFRSF1A ELISA Kit, Mouse

Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.

TNFRSF1A ELISA Kit, Mouse: 产品信息

产品名称
TNFRSF1A ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
5.60 pg/mL
线性范围
7.82-500 pg/mL
特异性
Recognizes both recombinant and natural Mouse TNFRSF1A
干扰
Preparations of mouse TNFa(80-235) at 5 ng/mL in a mid-range recombinant mouse TNFRSF1A control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 109.10 209.49 404.62
SD 2.77 4.94 14.11
CV(%) 2.5% 2.4% 3.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 104.90 203.65 384.84
SD 6.35 9.69 22.71
CV(%) 6.1% 4.8% 5.9%
回收率
The recovery of Mouse TNFRSF1A spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
94.7463524130191
Range
92-97%
线性关系
Serum
  Recovery of detected
1:2 86%
1:4 99%
1:8 110%
1:16 105%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This TNFRSF1A ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNFRSF1A protein in Serum . It contains recombinant Mouse TNFRSF1A, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TNFRSF1A ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse TNFRSF1A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TNFRSF1A ELISA Kit, Mouse: 别称

CD120a ELISA Kit, Mouse; FPF ELISA Kit, Mouse; p55 ELISA Kit, Mouse; p55-R ELISA Kit, Mouse; TNF-alphaR1 ELISA Kit, Mouse; TNF-R ELISA Kit, Mouse; TNF-R-I ELISA Kit, Mouse; TNF-R1 ELISA Kit, Mouse; TNF-R55 ELISA Kit, Mouse; TNFalpha-R1 ELISA Kit, Mouse; TNFAR ELISA Kit, Mouse; Tnfr-2 ELISA Kit, Mouse; Tnfr1 ELISA Kit, Mouse; TNFR60 ELISA Kit, Mouse; TNFRI ELISA Kit, Mouse; TNFRp55 ELISA Kit, Mouse

TNFRSF1A 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.
全称
tumor necrosis factor receptor superfamily member 1A
研究领域
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • TNF Signaling
    TNF Signaling
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.
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