VCAM1 ELISA Kit, Human

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VCAM1 ELISA Kit, Human: 产品信息

产品名称
VCAM1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant,Plasma
灵敏度
29.51 pg/mL
线性范围
109.38-7000 pg/mL
特异性
Recognizes both recombinant and natural Human VCAM1
干扰
Preparations of the factors listed below at 50 ng/m in a mid-range recombinant human VCAM1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 887.68 1,701.44 3,387.00
SD 69.76 115.30 296.82
CV(%) 7.9% 6.8% 8.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,217.22 2,368.75 4,637.27
SD 17.82 51.54 113.33
CV(%) 1.5% 2.2% 2.4%
回收率
The recovery of Human VCAM1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
89
Range
87 -90%
Sample
supernatant (n=3)
Average % Recovery
111
Range
101-127%
Sample
plasma (n=3)
Average % Recovery
86
Range
80-93%
线性关系
Serum
  Recovery of detected
1:2 100%
1:4 88%
1:8 87%
1:16 85%
Supernatant
  Recovery of detected
1:2 100%
1:4 90%
1:8 90%
1:16 87%
Plasma
  Recovery of detected
1:2 100%
1:4 98%
1:8 95%
1:16 89%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This VCAM1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human VCAM1 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human VCAM1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

VCAM1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human VCAM1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

VCAM1 ELISA Kit, Human: 别称

CD106 ELISA Kit, Human; INCAM-100 ELISA Kit, Human; VCAM-1 ELISA Kit, Human

VCAM1 背景信息

Vascular cell adhesion molecule 1 (VCAM-1), also known as CD16, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.
全称
vascular cell adhesion molecule 1
参考文献
  • Cook-Mills JM. (2002) VCAM-1 signals during lymphocyte migration: role of reactive oxygen species. Mol Immunol. 39(9): 499-508.
  • Preiss DJ, et al. (2007) Vascular cell adhesion molecule-1: a viable therapeutic target for atherosclerosis? Int J Clin Pract. 61(4): 697-701.
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