Vitronectin ELISA Kit, Human

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Vitronectin ELISA Kit, Human: 产品信息

产品名称
Vitronectin ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
15.19 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Human Vitronectin
干扰
Preparations of human SERPINE1 at 50 ng/mL in a mid-range recombinant Human Vitronectin control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 541.00 1,073.00 1,932.00
SD 28.36 66.63 140.49
CV(%) 5.2% 6.2% 7.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 514.67 995.00 1,845.00
SD 14.05 38.35 11.00
CV(%) 2.7% 3.9% 0.6%
回收率
The recovery of Human VTN spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
104
Range
96 -110%
线性关系
Serum
  Recovery of detected
1:2 104%
1:4 102%
1:8 98%
1:16 93%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Vitronectin ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Vitronectin protein in Serum . It contains recombinant Human Vitronectin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Vitronectin ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human Vitronectin. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Vitronectin ELISA Kit, Human: 别称

V75 ELISA Kit, Human; Vitronectin ELISA Kit, Human; VN ELISA Kit, Human; VNT ELISA Kit, Human

Vitronectin 背景信息

Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.
全称
vitronectin
参考文献
  • Ekmeki OB, et al. (2006) Vitronectin in atherosclerotic disease. Clin Chim Acta. 368(1-2): 77-83.
  • Derer W, et al. (2009) Vitronectin concentrations predict risk in patients undergoing coronary stenting. Circ Cardiovasc Interv. 2(1): 14-9.
  • Heyman L, et al. (2010) Mesothelial vitronectin stimulates migration of ovarian cancer cells. Cell Biol Int. 34(5): 493-502.
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