XPNPEP2 ELISA Kit, Human

Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.

XPNPEP2 ELISA Kit, Human: 产品信息

产品名称
XPNPEP2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
2.10 pg/mL
线性范围
9.38-600 pg/mL
特异性
Recognizes both recombinant and natural Human XPNPEP2
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 121.86 257.60 502.34
SD 3.98 6.85 13.86
CV(%) 3.3% 2.7% 2.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 119.36 246.49 493.16
SD 8.67 4.32 24.99
CV(%) 7.3% 1.8% 5.1%
回收率
The recovery of Human XPNPEP2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
94.4342395297575
Range
91-103%
线性关系
Serum
  Recovery of detected
1:2 103%
1:4 99%
1:8 94%
1:16 86%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This XPNPEP2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human XPNPEP2 protein in Serum . It contains recombinant Human XPNPEP2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

XPNPEP2 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human XPNPEP2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

XPNPEP2 ELISA Kit, Human: 别称

AEACEI ELISA Kit, Human; APP2 ELISA Kit, Human

XPNPEP2 背景信息

Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2, the X-linked gene that encodes membranous aminopeptidase P (APP), has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family, of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation, suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).
全称
X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound
参考文献
  • Sprinkle TJ, et al. (2000) Cloning, chromosomal sublocalization of the human soluble aminopeptidase P gene (XPNPEP1) to 10q25.3 and conservation of the putative proton shuttle and metal ligand binding sites with XPNPEP2. Arch Biochem Biophys. 378(1): 51-6.
  • Prueitt RL, et al. (2000) Physical mapping of nine Xq translocation breakpoints and identification of XPNPEP2 as a premature ovarian failure candidate gene. Cytogenet Cell Genet. 89(1-2): 44-50.
  • Duan QL, et al. (2005) A variant in XPNPEP2 is associated with angioedema induced by angiotensin I-converting enzyme inhibitors. Am J Hum Genet. 77(4): 617-26.
  • Woodard-Grice AV, et al. (2010) Sex-dependent and race-dependent association of XPNPEP2 C-2399A polymorphism with angiotensin-converting enzyme inhibitor-associated angioedema. Pharmacogenet Genomics. 20(9): 532-6.
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