Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit

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Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit: 产品信息

产品名称
Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
58.39 pg/mL
线性范围
78.13-5000 pg/mL
特异性
Recognizes both recombinant and natural ZIKV ZIKV NS-1 protein
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 975.42 2,004.10 4,161.34
SD 39.32 88.78 159.99
CV(%) 4.0% 4.4% 3.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,013.13 2,019.63 3,994.37
SD 52.03 59.02 153.37
CV(%) 5.1% 2.9% 3.8%
回收率
The recovery of ZIKV ZIKV-NS1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
100
Range
95-104%
线性关系
Supernatant
  Recovery of detected
1:2 101%
1:4 99%
1:8 95%
1:16 104%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of ZIKV ZIKV NS-1 protein protein in Recombinant protein . It contains recombinant ZIKV ZIKV NS-1 protein, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Zika virus (strain Zika SPH2015) ZIKV-NS1 ELISA Kit: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

ZIKV NS-1 protein 背景信息

Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
参考文献
  • Wong SS-Y, et al., Zika virus infectiondthe next wave after dengue?, Journal of the Formosan Medical Association (2016)
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