Flow Cytometry (FCM) / FACS Antibody

Flow Cytometry Antibody Search
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Strictly Validated Antibodies

Verified by Multiple Cell Samples
With an antibody validation through multiple cell lines will further clarify the antibody specificity.
Antigen Blocking Verified
This method is one of the most conventional antibody specificity verification methods.
Non-conjugated Antibody Blocking Verified
One purpose of this verification experiment with unlabeled antibodies is qualifying whether the specificity change after the labelling.
Fc Receptor Blocking Verified
It's always be recommended to do Fc receptors blockade prior to sample staining if your sample contains some special cell types.
WB,IF and IHC Verified
Other applications also provide information on specificity of the antibody.

Comparable with Classic Clone

The vast majority of monoclonal antibodies were comparable to the classic clones, which had good reputation internationally, in homogeneity, specificity, sensitivity, affinity, etc..

Excellent Performance in sensitivity

The antibody products in Sino Biological Inc. (SBI) have high affinity and sensitivity performance. They could be effective in very high dilution to recommended concentration in your experiments.

Verified High Stability

Antibody stability in Sino Biological Inc. (SBI) produced reagents is determined by real-time and real-temperature testing. We can certify the period of time that the antibody has been tested.

Excellent Performance in sensitivity

  Classic Clone            SB Clone
Recommended
Concentration
1:10
1:100

Verified High Stability

Conjugated Time 0
12 Month
24 Month
42 Month

Recommendations for Background Control

  • Unlabeled cells as blank control
  • Single color and FMO control
  • Isotype control
  • Negative cells control

Fluorochrome Selection

  • Excitation and Emission
  • Fluorochrome Selection

Flow Cytometry Tips

  • Sample preparation
  • Fluorescence staining

Flow Cytometry FAQ

  • What fluorochromes can I use?
  • What controls do I need?
  • Do the cells need to be permeabilized?
  • Do the samples need to be fixed?

Flow Cytometry PPT

  • Introduction to Flow Cytometry
  • Flow Cytometry Basic Training
  • Principles and Application of Flow Cytometry PowerPoint

Flow Cytometry Troubleshooting

  • No staining
  • Weak staining
  • Nonspecific staining
  • Unexpected staining

Flow Cytometry / FACS Background

Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection and protein engineering.

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