GLP Grade Antibody /Mab Production / Purification Service

All Antibody Services All Protein Services All Gene Services All Biological Services

Your Most Reliable & Cost-Effective Antibody R&D Partner

  3-8 wks   Risk-Free      
Send Mab Seq Receive Purified Mab Make Payment Upon Satisfaction   Inquire Now!
E-mail US
  No Upfront Payment!   No Risk to You!     You Order,We Deliver!

GLP Grade Antibody Production / Purification: High Monomer, Low Exdotoxin

Host Cells: HEK293 or CHO cell (please specify, cost for production in CHO cell is higher)
Technology: Recombinant technology, transient transfection, Serum-free cell culture
Purification: Affinity protein A/G/L chromatography column purification
Anion and Caton Exchange Chromatography &
Size Exclusion Chromatography Column (SEC) for antibody fragment
and aggregate removals to achieve high monomer purity
Endotoxin control throughout the entire production process
Deliverable: Purified antibodies meeting required QC specifications
(quantities to be specified by customer)
Quality Specification: >95-99% monomer antibody purity by HP-SEC & < 0.1 to 1 EU/mg Endotoxin
(cost and timeline may increase with increased monomer purity and lower endotoxin level
due to the need for process development, polishing, and QC testing)
Antibody Types: human, mouse, rat, rabbit, canine, rhesus IgG antibodies
- We have experience in producing IgA, IgM, and IgE antibodies
Applications: antibody discovery, antibody library, antibody engineering, Fab & ScFv conversion to Mab
suitable for antibody characterization, in vitro testing, in vivo testing
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

• > 300 Staff
• > 80 Bioreactors
• > 30 Purification System
• > 10,000 Mab Production
• > 5000 Protein Bulks
• > 5000 antibody development
• 1-100mg Mab Production in 3-6 weeks
• 1-10 g Mab Production in 6-8 weeks
• Preferred CRO Partner for Multiple Top-10 Pharmas

More Antibody /Mab Production Services

Antibody /Mab Purification Services

high monomer purity antibody production low endotoxin antibody production IgG Antibody Production & Purification IgA Antibody Production & Purification IgM Antibody Production & Purification Mouse IgG1 Antibody Purification Service Mouse IgG2a Antibody Purification Service Mouse IgG2b Antibody Purification Service Mouse IgG3 Antibody Purification Service Rat IgG Antibody Purification Service Rabbit Antibody Purification Service Polyclonal antibody purification by protein A/G Polyclonal antibody purification by antigen affinity column

Features of Antibody Production Services at Sino Biological

  • Record Speed in Antibody Production
  • From Mab Sequence to Purified
  • 1-100 mg Mab in 3-6 weeks
  • 1-10 gram Mab in 6-8 weeks
  • GLP Grade Antibody Production
  • High Monomer Purity: up to 99%
  • Low Endotoxin Level: up to 0.05 EU/mg
  • Unmatched Experience in Antibody Production
  • >gt;10,000 Mab Production
  • Almost 100% success rate
Cutting-Edge Technology Unlimited Capacity Unbelievable Cost-Saving

GLP Grade Antibody Production & Purification Service Description

In pre-clinical in vivo studies of therapeutic antibody candidates, it is essential that the antibody products used for animal studies be free of endotoxin and antibody aggregates. Therefore, it is always required to remove antibody fragments and aggregates generated in cell culture and downstream purification processes as well to remove endotoxin. Some antibodies are prone to aggregation, especially during elution from protein A/G column at low pH. Chromatographic purification steps are needed to remove aggregates and increase monomer antibody purity.

Endotoxin is another big challenge in pre-clinical production using transient transfection. This is because a large amount of expression plasmid is usually added to the cell culture and the plasmid has to be produced in E coli. fermentation. Endotoxin's property is quite similar to the DNA plasmid used for transient transfection. Therefore, one needs to remove as much as possible endotoxin from the DNA plasmid production and purification process, and control endotoxin level throughout the transient transfection and purification process to avoid introducing endotoxin into the process and removal any residual endotoxin from the antibody product. Experience and expertise is critical to achieve effective endotoxin control. In many cases, process development is needed to fine-tune process parameters for effective endotoxin removal.

Sino Biological has extensive experience in producing high-quality monoclonal antibodies meeting ...... <Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
- Antibody CRO Service Description
- Antibody Service Q&A
- Antibody /Mab Production CRO Service
- Antibody Purification Service
-- Antibody Purification Service Description
-- Antibody /Mab Production / Purification Service: High Monomer Purity
High Monomer Purity Antibody Purification Service Description
-- low endotoxin antibody production service
Low Endotoxin Antibody Purification Service Description
-- GLP Grade Antibody /Mab Production / Purification Service
GLP Grade Antibody Production Service Description
-- IgG Antibody /Mab Production & Purification Service
IgG Antibody Production & Purification Service Description
-- IgA Antibody/Mab Production & Purification Service
IgA Antibody Production & Purification Service Description
-- IgM Antibody /Mab Production / Purification Service
IgM Antibody Production & Purification Service Description
-- Mouse IgG1 Antibody /Mab Production / Purification Service
Mouse IgG1 IgG Antibody Production & Purification Service Description
-- Mouse IgG2a Antibody /Mab Production / Purification Service
Mouse IgG2a Antibody Production & Purification Service Description
-- Mouse IgG2b Antibody /Mab Production / Purification Service
Mouse IgG2b Antibody Production & Purification Service Description
-- Mouse IgG3 Antibody /Mab Production / Purification Service
Mouse IgG3 Antibody Production & Purification Service Description
-- Rat IgG Antibody /Mab Production / Purification Service
Rat IgG Antibody Production & Purification Service Description
-- Rabbit IgG Antibody /Mab Production / Purification Service
Rabbit IgG Antibody Production & Purification Service Description
-- Total Polyclonal Antibody Production / Purification Service
Total Polyclonal Antibody Production & Purification Service Description
-- Antigen-Specific Polyclonal Antibody Production / Purification Service
Antigen Specific Polyclonal Antibody Service Description
- Antibody Humanization Service / Services
- Custom Antibody Development Service / Services
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service