Goat Anti-Human IgG-Fc Secondary Antibody (Biotin)

 
 
Overview
 

Description:

Goat polyclonal Secondary Antibody to Human IgG-Fc (Biotin).

Host

Goat

Target species:

Human IgG-Fc

Specificity:

By ELISA this antibody reacts specifically with Human IgG-Fc.

 

Has cross-reactivity with rat IgG (>10%), rabbit IgG (5.3%), horse IgG (4.1%)

Minimal cross-reactivity with mouse IgG, bovine IgG, guinea pig IgG

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt.Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purity:

Immunogen affinity purified

Purification notes:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.2-5 μg/mL

ELISA

0.004-0.2 μg/mL

 WB

0.004-0.2 μg/mL

(Colormetric: 0.004-0.2 μg/mL; Chemiluminescent: 0.002-0.1 μg/mL)

IP

0.004-0.2 μg/mL

 
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