Goat Anti-Rabbit IgG Secondary Antibody (Biotin)

 
 
Overview
 

Description:

Goat polyclonal Secondary Antibody to Rabbit IgG (Biotin).

Host

Goat

Target species:

Rabbit IgG

Specificity:

By ELISA this antibody reacts specifically with Rabbit IgG.

 

Has cross-reactivity with guinea pig IgG (>10%), rat IgG (2.7%), human IgG (3.6%)

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Store at +4℃ short term (1-2 weeks). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt.Avoid repeated freeze-thaw cycles.

Constituents:

3% BSA, PBS, 0.1% Proclin300

Purity:

Immunogen affinity purified

Purification notes:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.1-4 μg/mL

ELISA

0.001-0.2 μg/mL

 WB

0.002-0.1 μg/mL

(Colormetric: 0.002-0.1 μg/mL; Chemiluminescent: 0.001-0.1 μg/mL)

IP

0.002-0.1 μg/mL

 
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