GSK3B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag

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GSK3B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
1263 bp
序列特征
Identical with the Gene Bank Ref. ID sequence (Nucleotide may contain silent mutation without changing amino acid sequence)
产品特征
Full length Clone DNA of Mus musculus glycogen synthase kinase 3 beta.
质粒
启动子
Enhanced CMV mammalian cell promoter
标签序列
GFPSpark Tag Sequence: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
pLen-F(CTCGTTTAGTGAACCGTCAGAATT), pLen-R(GAACCGGAACCCTTAAACATGT)
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
储存 & 运输
运输方式
Each tube contains 10μg lyophilized plasmid
储存条件
The lyophilized plasmid can be stored at room temperature for three months

GSK3B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag: 别称

7330414F15Rik cDNA ORF Clone, Mouse; 8430431H08Rik cDNA ORF Clone, Mouse; C86142 cDNA ORF Clone, Mouse; GSK-3 cDNA ORF Clone, Mouse; GSK-3beta cDNA ORF Clone, Mouse; GSK3 cDNA ORF Clone, Mouse

GSK3B 背景信息

GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.
全称
glycogen synthase kinase 3 beta
参考文献
  • Bergmann C, et al. (2011) Inhibition of glycogen synthase kinase 3β induces dermal fibrosis by activation of the canonical Wnt pathway. Ann Rheum Dis. 70(12):2191-8.
  • Ban JO, et al. (2011) Troglitazone, a PPAR agonist, inhibits human prostate cancer cell growth through inactivation of NFκB via suppression of GSK-3β expression. Cancer Biol Ther. 12(4):288-96.
  • Tsukigi M, et al. (2012) Re-expression of miR-199a suppresses renal cancer cell proliferation and survival by targeting GSK-3β. Cancer Lett. 315(2):189-97.
  • Nandan D, et al. (2012) Myeloid cell IL-10 production in response to leishmania involves inactivation of glycogen synthase kinase-3β downstream of phosphatidylinositol-3 kinase. J Immunol. 188(1):367-78.
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