Affinity purification by Glutathione Agarose Resin
Purified recombinant proteins (quantities to be specified by customer)
typically >90% purity by SDS page
Track Records & Key Facts
• > 300 Staff
• > 80 Bioreactors
• > 30 Purification System
• > 10,000 Mab Production
• > 5000 Protein Bulks
• > 5000 antibody development
• 1-100mg Mab Production in 3-6 weeks
• 1-10 g Mab Production in 6-8 weeks
• Preferred CRO Partner for Multiple Top-10 Pharmas
GST stands for Glutathione S-transferase. It is a family of eukaryotic and prokaryotic metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. It is typically fused to the N-terminus of a recombinant protein through recombinant DNA technology.
The peptide sequence of the GST-tag is 220 amino acids (roughly 26 KDa) in size.
Why use a GST-tag for protein expression and production?
Adding a GST-tag to a protein's N-terminus can ...... < Learn More>
GST-tag Protein Production & Purification Service Description
GST-fusion-tag (GST-fusion protein) has been widely used in recombinant protein expression and production. The fusion of the GST protein allows simple and efficient affinity purification of the GST-tagged protein using Glutathione affinity agarose resin. The GST-tag also can be used for detection in western blot by using a GST-tag-specific antibody. GST-tag is typically fused to the N-terminus of a protein for expression in virtually all expression systems, but for purification purposes, it is often used only in E. coli. expression system and baculovirus / insect cell expression system.
Sino Biological has successfully produced many proteins with the GST-tag and ...... < Learn More>