Her2 / ERBB2 Protein, Human, Recombinant (Fc Tag)

1/2
Price:
Size:
Number:

Her2 / ERBB2 Protein, Human, Recombinant (Fc Tag): 产品信息

纯度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its binding ability in a functional ELISA . Immobilized human ErbB2 at 2.5 μg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml .
蛋白构建
A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the Fc region of human IgG1 at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Thr 23
分子量
The recombinant human ErbB2/Fc chimera is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The monomer comprises 868 amino acids and has a calculated molecular mass of 96.1 kDa. As a result of glycosylation, the monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Her2 / ERBB2 Protein, Human, Recombinant (Fc Tag): 图片

Her2 / ERBB2 Protein, Human, Recombinant (Fc Tag): 别称

CD340 Protein, Human; ENV Protein, Human; ENVW Protein, Human; ERVWE1 Protein, Human; HER-2 Protein, Human; HER-2/neu Protein, Human; HER2 Protein, Human; HERV-7q Protein, Human; HERV-W-ENV Protein, Human; HERV7Q Protein, Human; HERVW Protein, Human; HERVWENV Protein, Human; MLN 19 Protein, Human; MLN19 Protein, Human; NEU Protein, Human; NGL Protein, Human; TKR1 Protein, Human

Her2 / ERBB2 背景信息

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
全称
erb-b2 receptor tyrosine kinase 2
参考文献
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
  • A new prodrug form of Affibody molecules (pro-Affibody) is selectively activated by cancer-associated proteases
    Author
    L Sandersjöö, A Jonsson, J Löfblom
    Year
    2014
    Journal
    Cellular and Molecular Life Sciences
    Application
    binding
  • Phage display as a tool for development of novel therapeutics for breast cancer
    Author
    Cabral, MJCD;
    Year
    2014
    Journal
    Thesis
    Application
    coating protein
  • Efficient production of Trastuzumab Fab antibody fragments in Brevibacillus choshinensis expression system
    Author
    Mizukami, M;Onishi, H;Hanagata, H;Miyauchi, A;Ito, Y;Tokunaga, H;Ishibashi, M;Arakawa, T;Tokunaga, M;
    Year
    2018
    Journal
    Protein Expr. Purif.
    Application
    ELISA
  • Harnessing iNKT and CD8 T cells for Immunotherapy of Cancer
    Author
    Tschumi, BO;
    Year
    2018
    Journal
    Thesis
    Application
    FAC (CAR-T)
  • High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles
    Author
    Longatti, A;Schindler, C;Collinson, A;Jenkinson, L;Matthews, C;Fitzpatrick, L;Blundy, M;Minter, R;Vaughan, T;Shaw, M;Tigue, N;
    Year
    2018
    Journal
    Nanoscale
    Application
    ELISA
  • Multiplex competitive analysis of HER2 and EpCAM cancer markers in whole human blood using Fe2O3@Ag nanocomposite
    Author
    Balzerová, A;Opletalová, A;Ranc, V;Zbořil, R;
    Year
    2018
    Journal
    Applied Materials Today
    Application
    binding
  • Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
    Author
    Harth, S;Ten Haaf, A;Loew, C;Frisch, C;Knappik, A;
    Year
    2018
    Journal
    MAbs
    Application
    ELISA
  • Site-specific conjugation of recognition tags to trastuzumab for Peptide Nucleic Acid-mediated radionuclide HER2 pretargeting
    Author
    Westerlund, K;Vorobyeva, A;Mitran, B;Orlova, A;Tolmachev, V;Karlström, A;Altai, M;
    Year
    2019
    Journal
    Biomaterials
    Application
    SPR
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后