PGK1 qPCR Primer Pairs, Human

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PGK1 qPCR Primer Pairs, Human: 基本信息

基因信息
种属:
Human
NCBI 参考序列号:
产物大小:
170bp
产品信息
寡核苷酸类型:
qPCR Primers
组分:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
qPCR 引物描述:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
应用 & 质控
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输方式:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存条件:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

特色与优势

独特的引物设计

在特定基因的不同变体保守区内设计引物,该对引物至少有一条引物跨越内含子或产物跨越内含子,有效避免基因组 DNA 的扩增。

严格的筛选验证过程

用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选,用阳性组织或细胞验证确认。

统一的 PCR 条件,操作方便,节约时间与成本

~100% 的扩增效率,保证 RNA 定量的准确性

PGK1 qPCR Primer Pairs, Human: 经验证的图片

PGK1 dissolution curves
PGK1 amplification curves

PGK1 qPCR Primer Pairs, Human: 别称

HEL-S-68p qPCR Primer Pairs, Human; MIG10 qPCR Primer Pairs, Human; PGKA qPCR Primer Pairs, Human

PGK1 背景信息

  • Epithelial cells release adenosine to promote local TNF production in response to polarity disruption
    Author
    Poernbacher, I;Vincent, JP;
    Year
    2018
    Journal
    Nat Commun
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