TGF beta 1 ELISA Kit, Human

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TGF beta 1 ELISA Kit, Human: 产品信息

产品名称
TGF beta 1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
23.2 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural Human TGF beta 1
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 529.00 1,063.00 2,115.00
SD 20.14 64.52 58.67
CV(%) 3.8% 6.1% 2.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 542.00 1,048.00 2,041.00
SD 18.36 16.09 85.22
CV(%) 3.4% 1.5% 4.2%
回收率
The recovery of Human TGFB1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Serum
  Recovery of detected
1:2 95%
1:4 105%
1:8 100%
1:16 92%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This TGF beta 1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 protein in Serum . It contains recombinant Human TGF beta 1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TGF beta 1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TGFβ1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TGF beta 1 ELISA Kit, Human: 别称

CED ELISA Kit, Human; DPD1 ELISA Kit, Human; LAP ELISA Kit, Human; TGF-beta 1 ELISA Kit, Human; TGFB ELISA Kit, Human; TGFbeta ELISA Kit, Human

TGF beta 1 背景信息

TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.
全称
transforming growth factor, beta 1
研究领域
参考文献
  • Ghadami M, et al. (2000) Genetic Mapping of the Camurati-Engelmann Disease Locus to Chromosome 19q13.1-q13.3. Am J Hum. Genet. 66(1):143-7.
  • Letterio J, et al. (1998) Regulation of immune responses by TGF-beta. Annu Rev Immunol. 16:137-61.
  • Vaughn SP, et al. (2000) Confirmation of the mapping of the Camurati-Englemann locus to 19q13. 2 and refinement to a 3.2-cM region. Genomics. 66(1):119-21.
  • Assoian R, et al. (1983) Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. J Biol Chem. 258(11):7155-60.
  • Influence of Th17/Treg balance on progression of liver cirrhosis in patients with chronic hepatitis B
    Author
    Wu, C;Dong, X;Yang, Q;Sun, Z;
    Year
    2018
    Journal
    International Journal of Clinical and Experimental Medicine
  • Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions
    Author
    Mu, Y;Wu, X;Hao, Z;
    Year
    2018
    Journal
    BMC Cell Biol.
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