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IgG antibodies are the most abundant antibody class in human and animal blood. IgG antibodies are most widely used as research reagents and also as therapeutic products. Human IgG antibodies are classified into four subtypes: IgG1, IgG2, IgG3, and IgG4. Mouse IgG antibodies are classified as IgG1, IgG2a, IgG2b, and IgG3.
IgG antibodies can be produced from hybridoma cell culture or with transient transfection technology in HEK293 cells or CHO cells. Human IgG antibodies binds well to protein A or protein G, so protein A affinity resin is widely used for human IgG antibody purification, regardless of the sub-class. However, for mouse and rat IgG antibodies, their binding affinity to protein A or G resin varies significantly. As a result, purifying some mouse and rat monoclonal antibodies could be a challenging task. Selection of the right resin and using appropriate purification buffers under optimal process conditions are critical for successful purification of some difficult-to-purify antibodies.
Sino Biological has well trained professional staff. It has produced and purified thousands of mouse and rat monoclonal antibodies from hybridoma cell culture as well as from transient transfection process. Our platform technology and experience can be of great value to you. By taking advantage of our antibody production and purification services, you can accelerate your antibody discovery and development projects while saving cost at the same time.
How to Submit a Request for Antibody Production and Purification Service?
If you are interested in using our antibody purification services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROemail@example.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.