IgM Antibody /Mab Production / Purification Service

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Your Most Reliable & Cost-Effective Protein & Antibody R&D Partner

• Cutting-edge technologies
• Most reliable partner
• Unmatched experience:>10,000 Mabs,>6000 Proteins

• Superior quality of products & services
• Unmatched speed of production
• Unmatched cost-saving

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IgM Antibody Production / Purification Service

Host Cells: HEK293 or CHO cell (please specify, cost for production in CHO cell is higher)
Mouse Hybridoma
Technology: Transient transfection or Stable CHO cell culture (please specify)
Serum-free cell culture, Fed-batch culture
IgM antibodies are expressed as a pentamer or hexamer linked by inter-molecule
disulfide bonds and a J-chain.
Purification: Anion and Caton Exchange Chromatography &
Size Exclusion Chromatography Column (SEC) for antibody fragment
and aggregate removals to achieve high monomer purity if needed
Protein A/G/L affinity purification cannot be used
Deliverable: Purified IgM antibodies meeting required QC specifications
(quantities to be specified by customer)
Quality Specification: antibody monomer purity and endotoxin level can be controlled to meet customer spec.
(cost and timeline may increase with increased monomer purity and lower endotoxin level
due to the need for process development, polishing, and QC testing)
Antibody Types: human, mouse, rat IgM antibodies
Applications: antibody engineering, Fab & ScFv conversion to Mab
suitable for antibody characterization, in vitro testing, in vivo testing
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

• > 300 Staff
• > 80 Bioreactors
• > 30 Purification System
• > 10,000 Mab Production
• > 5000 Protein Bulks
• > 5000 antibody development
• 1-100mg Mab Production in 3-6 weeks
• 1-10 g Mab Production in 6-8 weeks
• Preferred CRO Partner for Multiple Top-10 Pharmas

More Antibody /Mab Production Services

Antibody /Mab Purification Services

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Features of Antibody Production Services at Sino Biological

  • Record Speed in Antibody Production
  • From Mab Sequence to Purified
  • 1-100 mg Mab in 3-6 weeks
  • 1-10 gram Mab in 6-8 weeks
  • GLP Grade Antibody Production
  • High Monomer Purity: up to 99%
  • Low Endotoxin Level: up to 0.05 EU/mg
  • Unmatched Experience in Antibody Production
  • >gt;10,000 Mab Production
  • Almost 100% success rate
Cutting-Edge Technology Unlimited Capacity Unbelievable Cost-Saving

IgM Antibody Production & Purification Service Description

IgM antibody usually forms pentamer and hexamer polymers. Monomer IgM antibodies are covalently linked together with disulfide bonds. In pentameric IgM molecules, a J chain is present to hold two IgM molecules together with disulfide bonds. However, in the hexameric form, there is no J chain present.

Due to the large molecule weight and involvement of IGM light chain, heavy chain, and the J chain genes, recombinant IgM expression and production is rarely practiced. IgM antibody purification is particularly challenging because IgM antibody does not bind well to either protein A or protein G, and hence affinity purification is often not an option. In combination of low expression yield, large molecule weight, and poor stability, IgM antibody production seems to be an impossible task for most customers. However, IgM antibody has an advantage to IgG antibodies because of the pentamer structure of the antibody, meaning one IgM molecule could potentially binds to 10 copies of the antigen. As a result, IgM antibody sometime shows higher binding capability to IgG antibodies, especially for non-protein type of antigen and epitopes.

Sino Biological has devoted significant resources in developing in-house expertise in recombinant IgM expression, production, and purification. With our advanced mammalian transient transfection platform technology, we have achieved reasonable IgM expression in i...... <Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
- Antibody CRO Service Description
- Antibody Service Q&A
- Antibody /Mab Production CRO Service
- Antibody Purification Service
-- Antibody Purification Service Description
-- Antibody /Mab Production / Purification Service: High Monomer Purity
High Monomer Purity Antibody Purification Service Description
-- low endotoxin antibody production service
Low Endotoxin Antibody Purification Service Description
-- GLP Grade Antibody /Mab Production / Purification Service
GLP Grade Antibody Production Service Description
-- IgG Antibody /Mab Production & Purification Service
IgG Antibody Production & Purification Service Description
-- IgA Antibody/Mab Production & Purification Service
IgA Antibody Production & Purification Service Description
-- IgM Antibody /Mab Production / Purification Service
IgM Antibody Production & Purification Service Description
-- Mouse IgG1 Antibody /Mab Production / Purification Service
Mouse IgG1 IgG Antibody Production & Purification Service Description
-- Mouse IgG2a Antibody /Mab Production / Purification Service
Mouse IgG2a Antibody Production & Purification Service Description
-- Mouse IgG2b Antibody /Mab Production / Purification Service
Mouse IgG2b Antibody Production & Purification Service Description
-- Mouse IgG3 Antibody /Mab Production / Purification Service
Mouse IgG3 Antibody Production & Purification Service Description
-- Rat IgG Antibody /Mab Production / Purification Service
Rat IgG Antibody Production & Purification Service Description
-- Rabbit IgG Antibody /Mab Production / Purification Service
Rabbit IgG Antibody Production & Purification Service Description
-- Total Polyclonal Antibody Production / Purification Service
Total Polyclonal Antibody Production & Purification Service Description
-- Antigen-Specific Polyclonal Antibody Production / Purification Service
Antigen Specific Polyclonal Antibody Service Description
- Antibody Humanization Service / Services
- Custom Antibody Development Service / Services
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service