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IgM antibody usually forms pentamer and hexamer polymers. Monomer IgM antibodies are covalently linked together with disulfide bonds. In pentameric IgM molecules, a J chain is present to hold two IgM molecules together with disulfide bonds. However, in the hexameric form, there is no J chain present.
Due to the large molecule weight and involvement of IGM light chain, heavy chain, and the J chain genes, recombinant IgM expression and production is rarely practiced. IgM antibody purification is particularly challenging because IgM antibody does not bind well to either protein A or protein G, and hence affinity purification is often not an option. In combination of low expression yield, large molecule weight, and poor stability, IgM antibody production seems to be an impossible task for most customers. However, IgM antibody has an advantage to IgG antibodies because of the pentamer structure of the antibody, meaning one IgM molecule could potentially binds to 10 copies of the antigen. As a result, IgM antibody sometime shows higher binding capability to IgG antibodies, especially for non-protein type of antigen and epitopes.
Sino Biological has devoted significant resources in developing in-house expertise in recombinant IgM expression, production, and purification. With our advanced mammalian transient transfection platform technology, we have achieved reasonable IgM expression in HEK293 cells. In addition, we have developed highly effective chromatographic purification and polishing steps for IgM recovery and purification. We were able to obtain high purity IgM antibodies. We are starting to offer our expertise in this field to support our customer's recombinant IgM production and purification needs. If you have a need to produce and purify IgM antibodies, please let an expert like us to handle the task for you.
How to Submit a Request for Antibody Production and Purification Service?
If you are interested in using our antibody purification services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROemail@example.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.