Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit

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Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit: 产品信息

产品名称
Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
10 pg/mL
线性范围
46.88-3000 pg/mL
特异性
Recognizes both recombinant and natural H1N1 Hemagglutinin / HA
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.48 1.00 2.10
SD 0.01 0.03 0.05
CV(%) 2.4% 3.2% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.39 0.75 1.55
SD 0.05 0.09 0.20
CV(%) 12.9% 11.9% 12.9%
回收率
The recovery of H1N1 HA spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
89
Range
75 -97%
线性关系
Supernatant
  Recovery of detected
1:2 100%
1:4 101%
1:8 98%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of H1N1 Hemagglutinin / HA protein in Recombinant protein . It contains recombinant H1N1 Hemagglutinin / HA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Kit: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Hemagglutinin / HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
全称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
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