Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

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Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set 产品信息

产品名称
Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Influenza Hemagglutinin / HA
线性范围
15.63-1000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 1 mg/mL of mouse anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # CTA01)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 35 ng of recombinant Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
产品概述
This Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of Influenza Hemagglutinin / HA . It contains Influenza Hemagglutinin / HA capture antibody, Influenza Hemagglutinin / HA detector antibody (HRP) and a highly purified HEK293-expressed recombinant Influenza Hemagglutinin / HA protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set 图片

Hemagglutinin / HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
全称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
  • Microfluidic In-Reservoir Pre-concentration using a Buffer Drain Technique
    Author
    Yoon, J;Cho, Y;Han, S;Lee, J;Chung, S;
    Year
    2014
    Journal
    Lab on a Chip
    Application
    ELISA
  • Removal of bacterial suspension water occupying the intercellular space of detached leaves after agroinfiltration improves the yield of recombinant hemagglutinin in a Nicotiana benthamiana transient gene expression system
    Author
    Fujiuchi, N;Matsuda, R;Matoba, N;Fujiwara, K;
    Year
    2015
    Journal
    Biotechnol. Bioeng.
    Application
    ELISA
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