Interferon Gamma ELISA Kit, Human
Cat: KIT11725A
Interferon Gamma ELISA Kit, Human: 产品信息
产品名称
Interferon Gamma ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Cell supernatant
灵敏度
6.6 pg/mL
线性范围
15.63-1000 pg/mL
特异性
Recognizes both recombinant and natural Human Interferon Gamma
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human γ-IFN control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 142.45 | 285.86 | 549.84 |
| SD | 3.79 | 7.83 | 16.19 |
| CV(%) | 2.7% | 2.7% | 2.9% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 136.97 | 278.91 | 545.81 |
| SD | 13.49 | 19.67 | 28.79 |
| CV(%) | 9.8% | 7.1% | 5.3% |
回收率
The recovery of Human IFNG spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| supernatant (n=3) |
| Average % Recovery |
| 106 |
| Range |
| 102-115% |
线性关系
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 100% |
| 1:4 | 100% |
| 1:8 | 95% |
| 1:16 | 74% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Interferon Gamma ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon Gamma protein in Cell supernatant . It contains recombinant Human Interferon Gamma, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
Interferon Gamma ELISA Kit, Human: 图片
This assay recognizes both recombinant and natural Human γ-IFN. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Interferon Gamma ELISA Kit, Human: 别称
IFG ELISA Kit, Human; IFI ELISA Kit, Human; IFN gamma ELISA Kit, Human; Interferon Gamma ELISA Kit, Human
Interferon Gamma 背景信息
IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
全称
interferon, gamma
研究领域
- Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Flaviviridae>>Dengue virus (DENV)>>DENV infection associated>>DENV infection related
- Immunology>>Inflammation / Inflammatory Mediator>>Inflammatory Disorders Therapeutic Targets>>Inflammatory Bowel Diseases Therapeutic Targets
- Cardiovascular>>Angiogenesis>>Cytokine & Receptor>>Interferon & Receptor>>Interferon
- Immunology>>Innate Immunity>>Cytokine>>Interferons
- Stem Cells>>Stem Cell Growth Factors,Chemokines and Cytokines>>Mesenchymal Stem Cells (MSCs) Growth Factors, Chemokines and Cytokines
- Stem Cells>>Stem Cell Growth Factors,Chemokines and Cytokines>>Neural Stem Cells (NSCs) Growth Factors, Chemokines and Cytokines
- Immunology>>Inflammation / Inflammatory Mediator>>Neuroinflammation
参考文献
- Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
- Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
- Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
- Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
- Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
- Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.
Related Pathways
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IL12 signaling pathway
