Anti-ARF3 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-ARF3 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-ARF3 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-ARF3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-ARF3 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-ARF3 magnetic Beads, conjugated with Anti-ARF3 antibody, are used for immuneprecipitation (IP) of ARF3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ARF3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ARF3 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-ARF3 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-ARF3 Antibody, Rabbit Polyclonal(14967-T44)
免疫原
Recombinant Human ARF3 Protein (Catalog#14967-H07E)
交叉反应
Human ARF3
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Human ARF3 (rh ARF3; Catalog#14967-H07E; P61204; Met1-Lys181). ARF3 specific IgG was purified by Human ARF3 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

ARF3 背景信息

ARF3, also known as ADP-ribosylation factor 3, belongs to the RAS superfamily. Members of this family include ARF1, ARF2, ARF3, ARF4, ARF5 and ARF6. ARF3 gene is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin subunit, an ADP-ribosyltransferase. It is involved in protein trafficking and may modulate vesicle budding and uncoating within the Golgi apparatus.
全称
ADP-ribosylation factor 3
参考文献
  • Hirai M. et al., 1997, Genomics. 34 (2): 263-5.
  • Kanoh H. et al., 1997, J Biol Chem. 272 (9): 5421-9.
  • Boman. et al., 2002, Mol Biol Cell. 13 (9): 3078-95.
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