Anti-CD13 Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.
Price:
Size:
Number:

Anti-CD13 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD13 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD13 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD13 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD13 magnetic Beads, conjugated with Anti-CD13 antibody, are used for immuneprecipitation (IP) of CD13 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD13 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD13 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD13 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-CD13 Antibody, Rabbit Polyclonal(10051-T60)
免疫原
Recombinant Human CD13 / ANPEP Protein (Catalog#10051-H08H)
交叉反应
Human CD13 / ANPEP
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Human CD13 / ANPEP (rh CD13 / ANPEP; Catalog#10051-H08H; NP_001141.2; Lys69-Lys967,603Met/Ile). CD13 / ANPEP specific IgG was purified by Human CD13 / ANPEP affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD13 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-APNALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CD13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-GP150ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LAP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-P150ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PEPNALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD13 背景信息

Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.
全称
alanyl (membrane) aminopeptidase
参考文献
  • Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.
  • Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.
  • Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.