Anti-CD166/ALCAM Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD166/ALCAM 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD166/ALCAM Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD166/ALCAM magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD166/ALCAM 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD166/ALCAM magnetic Beads, conjugated with Anti-CD166/ALCAM antibody, are used for immuneprecipitation (IP) of CD166/ALCAM proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD166/ALCAM proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD166/ALCAM proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD166/ALCAM 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-CD166/ALCAM Antibody, Rabbit Polyclonal(90227-T52)
免疫原
Recombinant Cynomolgus CD166 / ALCAM protein (Catalog#90227-C08H)
交叉反应
Cynomolgus CD166 / ALCAM
来源
Polyclonal Cynomolgus Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Cynomolgus CD166 / ALCAM (Catalog#90227-C08H; G7NZQ8; Met1-Ala526). CD166 / ALCAM specific IgG was purified by Cynomolgus CD166 / ALCAM affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

CD166/ALCAM 背景信息

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.
全称
activated leukocyte cell adhesion molecule
参考文献
  • Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.
  • Fujiwara H, et al. (2003) Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 88(7): 3437-43.
  • Jezierska A, et al. (2006) ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12(8): BR263-73.
  • Kahlert C, et al. (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101(3): 457-64.
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