Anti-CD22 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD22 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD22 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD22 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD22 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD22 magnetic Beads, conjugated with Anti-CD22 antibody, are used for immuneprecipitation (IP) of CD22 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD22 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD22 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD22 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Recombinant Anti-CD22 Antibody, Rabbit Monoclonal(51177-R087)
免疫原
Recombinant Mouse CD22 Protein (Catalog#51177-M08H)
交叉反应
Mouse CD22
来源
Monoclonal Mouse Rabbit IgG
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD22 (rM CD22; Catalog#51177-M08H; BAE33829.1; Met1-Arg708).
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD22 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-A530093D23ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Lyb-8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Lyb8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD22 背景信息

CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.
全称
CD22 molecule
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
参考文献
  • Tedder TF, et al. (1997) CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504.
  • Tedder TF, et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
  • Fujimoto M, et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
  • Walker JA, et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25.
  • Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.
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