Anti-CD38 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD38 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD38 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD38 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD38 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD38 magnetic Beads, conjugated with Anti-CD38 antibody, are used for immuneprecipitation (IP) of CD38 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD38 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD38 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD38 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-CD38 Antibody, Rabbit Polyclonal(10818-T60)
免疫原
Recombinant Human CD38 Protein (Catalog#10818-H08H)
交叉反应
Human CD38
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Human CD38 (rh CD38; Catalog#10818-H08H; NP_001766.2; Val43-Ile300). CD38 specific IgG was purified by Human CD38 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD38 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-ADPRC 1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ADPRC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-T10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD38 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.
全称
CD38 molecule
研究领域
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
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