Anti-CD53 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD53 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD53 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD53 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD53 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD53 magnetic Beads, conjugated with Anti-CD53 antibody, are used for immuneprecipitation (IP) of CD53 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD53 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD53 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD53 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Recombinant Anti-CD53 Antibody, Rabbit Monoclonal(50722-R004)
免疫原
Recombinant Mouse CD53 Protein (Catalog#50722-M07H)
交叉反应
Mouse CD53
来源
Monoclonal Mouse Rabbit IgG
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD53 (rM CD53; Catalog#50722-M07H; Q61451; Glu107-Asn181).
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD53 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-AI323659ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Ox-44ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Tspan25ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD53 背景信息

CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.
全称
CD53 molecule
参考文献
  • Rochelle JM, et al. (1993) Gene structure, chromosomal localization, and protein sequence of mouse CD53 (Cd53): evidence that the transmembrane 4 superfamily arose by gene duplication. Int Immunol. 5(2):209-16.
  • Virtaneva KI, et al. (1993) The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics. 37(6):461-5.
  • Horejsí V, et al. (1991) Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS Lett. 288(1-2):1-4.
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