Anti-CD73 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD73 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD73 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD73 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD73 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD73 magnetic Beads, conjugated with Anti-CD73 antibody, are used for immuneprecipitation (IP) of CD73 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD73 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD73 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD73 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-CD73 Antibody, Rabbit Polyclonal(80335-T60)
免疫原
Recombinant Rat CD73/NT5E Protein (Catalog#80335-R08H)
交叉反应
Rat CD73/NT5E
来源
Polyclonal Rat Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Rat CD73/NT5E (rR CD73/NT5E; Catalog#80335-R08H; Q66HL0; Met1-Lys549). CD73/NT5E specific IgG was purified by Rat CD73/NT5E affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

CD73 背景信息

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
全称
5'-nucleotidase, ecto (CD73)
参考文献
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
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