Anti-CD86 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD86 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CD86 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD86 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD86 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CD86 magnetic Beads, conjugated with Anti-CD86 antibody, are used for immuneprecipitation (IP) of CD86 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD86 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD86 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD86 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-CD86 Antibody, Rabbit Polyclonal(50068-RP02)
免疫原
Recombinant Mouse CD86 protein (Catalog#50068-M08H)
交叉反应
Mouse CD86 / ETC-1 / B7-2
来源
Polyclonal Mouse Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). CD86 specific IgG was purified by mouse CD86 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD86 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-B7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-B7-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-B7.2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-B70ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Cd28l2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CLS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ETC-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Ly-58ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Ly58ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MB7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MB7-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TS/A-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD86 背景信息

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
全称
CD86 antigen
研究领域
参考文献
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
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