Anti-CTLA-4 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CTLA-4 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-CTLA-4 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CTLA-4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CTLA-4 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-CTLA-4 magnetic Beads, conjugated with Anti-CTLA-4 antibody, are used for immuneprecipitation (IP) of CTLA-4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CTLA-4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CTLA-4 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CTLA-4 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-CTLA-4 Antibody, Rabbit Polyclonal(50503-RP02)
免疫原
Recombinant Mouse CTLA-4 / CD152 protein (Catalog#50503-M08H)
交叉反应
Mouse CTLA-4 / CD152
来源
Polyclonal Mouse Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Mouse CTLA-4 / CD152 (rM CTLA-4 / CD152; Catalog#50503-M08H; NP_033973.2; Met 1-Phe 162). CTLA-4 / CD152 specific IgG was purified by Mouse CTLA-4 / CD152 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CTLA-4 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-Cd152ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Ctla-4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Ly-56ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CTLA-4 背景信息

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
全称
cytotoxic T-lymphocyte-associated protein 4
参考文献
  • Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.
  • Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.
  • Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.
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